Studies of Polypeptide Structure by Fluorescence Techniques

Structure-activity studies of vasoactive intestinal polypeptide.

This report explores the potential side-chain functional groups required for interaction of the bronchodilator neuropeptide, vasoactive intestinal peptide (VIP), with its receptor. The binding affinity and biological activity of native VIP have been found to be sensitive to the removal of amino- and carboxyl-terminal residues. This data suggests that elements within the entire primary sequence ...

Dynamic structure of lipid bilayers studied by nanosecond fluorescence techniques.

Molecular motions in liposomes of dipalmitoyl-phosphatidylcholine (DPPC) were studied by nanosecond fluorescence techniques. As a fluorescent probe for the hydrocarbon region, 1,6-diphenyl-1,3,5-hexatriene (DPH) was used. Time courses of fluorescence intensity IT(t) and emission anisotropy r(t) of DPH embedded in DPPC liposomes were measured at various temperatures. The value of the fluorescenc...

Surface Plasmon Fluorescence Techniques for Bioaffinity Studies

Among the various sensing principles proposed for bioaffinity studies, optical evanescent wave techniques have gained the lead in popularity. Next to evanescent ellipsometry [1] and the various optical waveguide platforms [2,3], surface plasmon resonance (SPR) spectroscopy [4–6], in particular, has found widespread applications and has demonstrated its potential for the sensitive detection of b...

the role of task-based techniques on the acquisition of english language structures by the intermediate efl students

this study examines the effetivenss of task-based activities in helping students learn english language structures for a better communication. initially, a michigan test was administered to the two groups of 52 students majoring in english at the allameh ghotb -e- ravandi university to ensure their homogeneity. the students scores on the grammar part of this test were also regarded as their pre...

The polypeptide structure of immunoglobulin: experimental studies on the recombination of radiolabelled myeloma polypeptide chains.